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Test Name

Blood Culture

Test Code

BCULT1

Specimen Type

Ìý
• Yellow paediatric bottle – Neonates and Infants (<3 Years), 0.5-4mL
• Green aerobic bottle –ÌýChildren and Adults, 5-10mL
• Orange anaerobic bottle - Children and Adults, 5-10mL
• Mycoplasma Bottle (Silver cap) - Women (RWH), 3-5mL
  
• Mycobacterial bottle (Myco/F Lytic) (Red cap) 1-5 mL

Minimum Volume

Paediatric (yellow cap): 0.5mL blood; Aerobic (green cap): 5mL blood; Anaerobic (orange cap): 5mL blood; Mycoplasma (silver cap): 3mL blood; Mycobacterial bottle (Myco/F Lytic): 1mL

Preferred Volume

Paediatric (yellow cap): 4mL blood; Aerobic (green cap): 10mL blood; Anaerobic (orange cap): 10mL blood; Mycoplasma (silver cap): 5mL blood; Mycobacterial bottle (Myco/F Lytic): 5mL

Comments

Blood Culture bottle labelling

Specimen identification labels are to be placed flat as displayed below.Ìý Label must not obscure the bottle barcode or volume window.

Laboratory notes

• Each site must have separate laboratory number
• Each laboratory number can have up to 2 blood culture bottles e.g. PF Plus and FA Plus/ FN Plus (mycoplasma bottle not included)
• If 2 bottles are received (same colour) but 2 different sites

Ìý Ìý Ìý – enter as TWO separate lab numbers. Write the corresponding site on the Bottle ID barcode label on the request slip.

• If two different coloured bottles received (same site) – these are to be entered on ONE laboratory number.
• Peel the detachable tag of the Bottle ID barcode and affix to the request slip.
• Register the specimen to access the Laboratory number barcode (aliquot label) in central specimen reception.
• Affix the aliquot label containing the Laboratory number (LN) barcode at the bottom of the patient identifier in a vertical orientation. The lab number barcode and the bottle barcode orientation must be the same.
• After hours, place registered blood culture bottles on the VIRTUOâ„¢ as soon as possible by Core Lab staff.
• ÌýDo NOT store in incubator or leave on the bench at room temperature
  

Assay Performed

Bacteriology

RCH

Phone: 9345 5739

Laboratory Hours: Mon-Fri: 08.45 - 24.00hrs

Weekends & Public Holidays: 08.30 - 24.00hrs AFTER HOURS: On-Call Scientist

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Assay Frequency

When received.ÌýExpected turnaround 2-5days

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